SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : IV

Vail #

: 9

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin011.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 8:14:18 AM : 1/23/2007 2:02:00 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin011.lcd

mV

1Det.A Ch1

750

500

4

6

5

3

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.523 19.708 20.374 21.391 22.255 25.544

651382 586819

103380 83106 327201 413032 354194 374002 1654916

6.367 5.736

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

2231492 2472835 2130939 2157826 10231294

21.810 24.169 20.828 21.090 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin011.lcd