SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : IV

Vail #

: 9

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin012.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 9:00:04 AM : 1/23/2007 2:02:33 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin012.lcd

mV

1Det.A Ch1

750

500

4

6

5

3

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.533 19.682 20.351 21.367 22.230 25.520

651009 585559

103534 83536 327740 414098 353707 374004 1656618

6.370 5.729

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

2227105 2469751 2130222 2156794 10220438

21.791 24.165 20.843 21.103 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin012.lcd

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