SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : IV

Vail #

: 9

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin015.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 11:17:23 AM : 1/23/2007 2:04:54 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin015.lcd

mV

1Det.A Ch1

750

500

4

6

5

3

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.520 19.680 20.349 21.370 22.235 25.530

651869 584964

105067 83301 326954 414982 352505 374085 1656894

6.377 5.722

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

2224755 2471248 2131439 2158084 10222360

21.764 24.175 20.851 21.111 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin015.lcd