SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : V

Vail #

: 8

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin006.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 4:25:32 AM : 1/23/2007 1:54:34 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin006.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.499 19.657 20.328 21.351 22.216 25.509

329878 298008 1134850 1256078 1084798 1095189 5198801

52391 42420 167103 210138 179593 189856 841502

6.345 5.732

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.829 24.161 20.866 21.066 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin006.lcd