SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : V

Vail #

: 8

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin007.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 5:11:18 AM : 1/23/2007 1:55:57 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin007.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.479 19.664 20.332 21.358 22.226 25.526

330019 297953 1134742 1254992 1084624 1094573 5196904

53099 42274 166376 209684 179021 189990 840443

6.350 5.733

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.835 24.149 20.871 21.062 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin007.lcd