SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : V

Vail #

: 8

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin010.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 7:28:32 AM : 1/23/2007 2:00:32 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin010.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.506 19.664 20.336 21.358 22.224 25.520

329140 297517 1131663 1254538 1082926 1093649 5189432

52333 42403 166080 209221 179282 189781 839100

6.343 5.733

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.807 24.175 20.868 21.075 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin010.lcd