SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : VI

Vail #

: 7

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin003.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 2:08:21 AM : 1/23/2007 1:49:22 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin003.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.475 19.658 20.327 21.357 22.224 25.515

166015 151156 573669 634904 550930 552601 2629275

26501 21357 84147 106253 91150 96154 425561

6.314 5.749

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.819 24.148 20.954 21.017 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin003.lcd