SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : VI

Vail #

: 7

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin005.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 3:39:50 AM : 1/23/2007 1:52:14 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin005.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.483 19.665 20.333 21.356 22.221 25.509

165993 150985 572942 634615 550635 552430 2627600

26475 21409 83865 106347 91431 95695 425221

6.317 5.746

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.805 24.152 20.956 21.024 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin005.lcd