SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix : Stock solution

Sample ID

Vail #

: 13

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin031.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 11:37:48 PM : 1/23/2007 2:33:30 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin031.lcd

mV

2500

1Det.A Ch1

3

4

5

2000

6

1500

1

2

1000

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 9597190 8731747 21469032 18080882 13878628 12264511 84021990

Height

Area %

Name

1 2 3 4 5 6 Total

15.513 19.688 20.337 21.360 22.219 25.521

1502923 1218885 2361628 2312241 2099972 1984228 11479877

11.422 10.392 25.552 21.519 16.518 14.597 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin031.lcd