SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix : Stock solution

Sample ID

Vail #

: 13

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin032.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 12:23:36 AM : 1/23/2007 2:34:01 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin032.lcd

mV

1Det.A Ch1

3

4

5

2000

6

1500

1

2

1000

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 9587035 8721728 21438480 18085009 13809664 12206630 83848546

Height

Area %

Name

1 2 3 4 5 6 Total

15.516 19.695 20.344 21.365 22.224 25.527

1490793 1218646 2361255 2311064 2092715 1974740 11449213

11.434 10.402 25.568 21.569 16.470 14.558 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin032.lcd