SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix : Stock solution

Sample ID

Vail #

: 13

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin035.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 2:40:53 AM : 1/23/2007 2:43:56 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin035.lcd

mV

1Det.A Ch1

3

4

5

2000

6

1500

1

2

1000

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 9579369 8714428 21442185 17998894 13664575 12051739 83451189

Height

Area %

Name

1 2 3 4 5 6 Total

15.511 19.704 20.352 21.372 22.228 25.520

1496492 1219106 2360662 2309818 2082704 1961756 11430539

11.479 10.443 25.694 21.568 16.374 14.442 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin035.lcd