SPDS SET 2: ASH-01

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: 13.01.07

Vail #

: 40

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Std-rep005.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 7:15:23 AM : 3/29/2007 4:24:59 PM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-rep005.lcd

mV

1Det.A Ch1

1000

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Name

Area

Area %

Resolution

1 2 3 4 5 6 Total

15.516 19.692 20.366 21.379 22.242 25.539

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

1234072 391438 5318748 5001196 3786871 2839005 18571330

6.645 2.108

0.000 22.163 3.454 5.576 5.030 19.518

28.640 26.930 20.391 15.287 100.000

Withanollide A Withanolide B

Chromatogram D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-rep005.lcd

mV

1000

500

0

1Det.A Ch1

15.0

17.5

20.0

22.5

25.0

min

Det.A Ch1 / 227nm 1