SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1000 mg / 100 ml : Trl 1

Vail #

: 32

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP003.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 3:04:58 AM : 1/23/2007 3:10:30 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP003.lcd

mV

1Det.A Ch1

3

200

1

4

5

100

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.521 19.676 20.354 21.366 22.227 25.522

644576 539952 1477658 739704 520470 165582 4087942

114102 77457 208029 121587 93477 29605 644257

15.768 13.208 36.147 18.095 12.732 4.050 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP003.lcd