SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1500 mg / 100 ml : Trl 2

Vail #

: 35

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP006.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 5:22:10 AM : 1/23/2007 3:14:41 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP006.lcd

mV

1Det.A Ch1

400

3

300

1

4

200

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1029071 860618 2362250 1182463 843789 264250 6542442

Height

Area %

Name

1 2 3 4 5 6 Total

15.537 19.715 20.388 21.398 22.257 25.540

181569 123678 332899 194673 150165 47318 1030303

15.729 13.154 36.107 18.074 12.897 4.039 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP006.lcd