AOAC-RI ERP Micro June 2016

OMAMAN-29 A/ Collaboartive Study Manuscript OMA ERP June 2016 ERP Use Only

(n) Demi Fraser Broth - Available from 3M Food Safety (St. Paul, MN 55144-1000,

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USA).

(o) Ferric Ammonium Citrate (FAC), ACS grade, 5% sterilized - Available from MP

Biomedicals™or equivalent.

(p) Disposable pipette – capable of 20 µL (q) Multi - channel (8-channel) pipette - capable of 20 µL (r) Sterile filter tip pipette tips - capable of 20 µL (s) Filter Stomacher® bags – Seward or equivalent .

(t) Stomacher ®– Seward or equivalent. (u) Thermometer – calibrated range to include 100 ± 1 o C (v) Dry block heater unit– capable of maintaining 100 ± 1 o C (w) Incubators. – Capable of maintaining 37 ± 1°C or 41.5 ± 1°C. (x) Freezer – capable of maintaining -10 to -20 o C, for storing the 3M Molecular

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Detection Chill Block Tray

(y) Refrigerator – capable of maintaining 2-8 o C, for storing the 3M Molecular Detection

Assay components

(z) Computer – compatible with the 3M ™ Molecular Detection Instrument 3M™ Enviroswab, -- (hydrated with Letheen) Available from 3M Food (aa)

Safety(Australia)

(bb) 3M™ Hydrated Sponge Stick with 10 mL of D/E – Available from 3M Food

Safety(St. Paul, MN 55144-1000, USA)

C. General Instructions

(a) Store the 3M Molecular Detection Assay 2 - Listeria at 2-8°C. Do not freeze. Keep kit away from light during storage. After opening the kit, check that the foil pouch is undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes should always be stored in the re-sealable pouch with the desiccant inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 60 days. Do not use 3M Molecular Detection Assay 2 - Listeria past the (b) Follow all instructions carefully. Failure to do so may lead to inaccurate results. AOAC Research Institute Expert Review Panel Use Only Safety Precautions The 3M MDA2 – Listeria is intended for use in a laboratory environment by professionals trained in laboratory techniques. 3M has not documented the use of this product in industries other than the food and beverage industries. For example, 3M has not documented this product for testing drinking water, pharmaceutical, cosmetics, clinical or veterinary samples. The 3M MDA2 – Listeria has not been evaluated with all possible food products, food processes, testing protocols or with all expiration date.

possible strains of bacteria.

As with all test methods, the source of enrichment medium can influence the results. The 3M MDA 2 – Listeria has only been evaluated for use with the enrichment media

specified in the Instructions for Use section.

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