AOAC-RI ERP Micro June 2016

OMAMAN-29 A/ Collaboartive Study Manuscript OMA ERP June 2016 ERP Use Only

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Table 2: Enrichment protocols using Demi-Fraser Broth at 37 ± 1 °C according to AOAC Performance Tested SM Certificate #111501

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Enrichment Broth Volume (mL)

Enrichment Time (hr)

Sample Matrix

Sample Size

Beef hot dogs, Queso Fresco, Vanilla Ice Cream, 4% Milk Fat Cottage Cheese, 3% chocolate whole milk, romaine lettuce, bagged raw spinach, cold smoked salmon

25 g

225

24-30

Raw chicken

25 g

475

28-32

Deli turkey

125 g

1125

24-30

Enough volume to allow melon to float

Whole melon

26-30

Cantaloupe

Stainless steel Sealed concrete

1 sponge

225

24-30

1 sponge

100

24-30

Plastic AOAC Research Institute Expert Review Panel Use Only 1 swab 10 24-30

Environmental samples:

6 7 F. P REPARATION OF THE 3M™M OLECULAR D ETECTION H EAT B LOCK I NSERT 8 Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and set the temperature to allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of 100 ±1°C. NOTE: Depending on the heater unit, allow approximately 30minutes for the 3M Molecular Detection 12 Heat Block Insert to reach temperature. Using an appropriate, calibrated thermometer (e.g., a partial 13 9 10 11

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