AOAC-RI ERP Micro June 2016

OMAMAN-29 A/ Collaboartive Study Manuscript OMA ERP June 2016 ERP Use Only

Table 2016.1A :Summary of Results for the Detection of Listeria in Deli Turkey (125g)

1

Method a

3M ™ MDA ™ 2 - Listeria

Un-inoculated

Low

High

Inoculation Level

Candidate Presumptive Positive/ Total # of Samples Analyzed

0/132

68/132

132/132

0.00 (0.00, 0.03) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.00 (0.00, 0.03) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.00 (0.00, 0.03) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.00 (0.00, 0.03) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.00 (-0.03, 0.03) 0.00 (-0.03, 0.03) 1.0000 0/132 1.0000 0/132 1.0000 1.0000

0.52 (0.43, 0.60) 0.51 (0.45, 0.52) 0.00 (0.00, 0.15) 0.51 (0.46, 0.52) 0.50 (0.41, 0.59) 0.51 (0.46, 0.52) 0.00 (0.00, 0.14) 0.51 (0.46, 0.52) 0.50 (0.41, 0.59) 0.51 (0.46, 0.52) 0.00 (0.00, 0.14) 0.51 (0.46, 0.52) 0.45 (0.37, 0.54) 0.51 (0.46, 0.52) 0.00 (0.00, 0.11) 0.51 (0.46, 0.52) 0.04 (-0.08, 0.17) 0.02 (-0.11, 0.14) 0.8762 66/132 0.9123 66/132 0.9123 0.9829

1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 1.0000 132/132 1.0000 132/132 1.0000

Candidate Presumptive POD (CP)

b

s r

c

s L

d

s R

P Value e

Candidate Confirmed Positive/ Total # of Samples Analyzed

Candidate Confirmed POD (CC)

s r

s L

s R

P Value

Candidate Confirmed Positive/ Total # of Samples Analyzed Candidate Presumptive Positive that Confirmed POD (C)

s r

s L

s R

P Value

Positive Reference Samples/ Total # of Samples Analyzed AOAC Research Institute Expert Review Panel Use Only 0/132 60/132 132/132 Reference POD s r

s L

s R

P Value

1.0000

0.00 (-0.03, 0.03)

dLPOD (Candidate vs. Reference) f

dLPOD (Candidate Presumptive vs. Candidate Confirmed) f 0.00 (-0.03, 0.03) a Results include 95% Confidence Intervals, r Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation 2 e P Value = Homogeneity test of laboratory PODs; f A confidence interval for dLPOD that does not contain the value 0 indicates a statistical significant difference between 3 the two methods 4

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