AOAC-RI ERP Micro June 2016

OMAMAN-29 A/ Collaboartive Study Manuscript OMA ERP June 2016 ERP Use Only

Table 2016.1B :Summary of Results for the Detection of Listeria in Raw Chicken Breast Fillet (25g)

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Method a

3M ™ MDA ™ 2 - Listeria

Un-inoculated

Low

High

Inoculation Level

Candidate Presumptive Positive/ Total # of Samples Analyzed

2/132

88/132

131/132

0.02 (0.00, 0.06) 0.12 (0.11, 0.16) 0.00 (0.00, 0.05) 0.12 (0.11, 0.14) 0.01 (0.00, 0.04) 0.09 (0.08, 0.16) 0.00 (0.00, 0.04) 0.09 (0.08, 0.10) 0.01 (0.00, 0.04) 0.09 (0.08, 0.16) 0.00 (0.00, 0.04) 0.09 (0.08, 0.10) 0.00 (0.00, 0.03) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.01 (-0.02, 0.04) 0.01 (-0.03, 0.05) 0.5190 1/132 0.4338 1/132 0.4338 1.0000

0.67 (0.58, 0.75) 0.48 (0.42, 0.52) 0.00 (0.00, 0.18) 0.48 (0.43, 0.52) 0.65 (0.57, 0.73) 0.48 (0.43, 0.52) 0.00 (0.00, 0.18) 0.48 (0.43, 0.52) 0.64 (0.56, 0.73) 0.48 (0.43, 0.52) 0.00 (0.00, 0.18) 0.49 (0.43, 0.52) 0.48 (0.40, 0.57) 0.51 (0.46, 0.52) 0.00 (0.00, 0.14) 0.51 (0.46, 0.52) 0.16 (0.04, 0.28) 0.02 (-0.10, 0.13) 0.6044 86/132 0.5632 85/132 0.6228 0.9192

0.99 (0.96, 1.00) 0.09 (0.08, 0.16) 0.00 (0.00, 0.04) 0.09 (0.08, 0.10) 1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.99 (0.96, 1.00) 0.09 (0.08, 0.16) 0.00 (0.00, 0.04) 0.09 (0.08, 0.10) 1.00 (0.97, 1.00) 0.00 (0.00, 0.16) 0.00 (0.00, 0.16) 0.00 (0.00, 0.23) 0.4338 132/132 1.0000 131/132 0.4338

Candidate Presumptive POD (CP)

b

s r

c

s L

d

s R

P Value e

Candidate Confirmed Positive/ Total # of Samples Analyzed

Candidate Confirmed POD (CC)

s r

s L

s R

P Value

Candidate Confirmed Positive/ Total # of Samples Analyzed Candidate Presumptive Positive that Confirmed POD (C)

s r

s L

s R

P Value

Positive Reference Samples/ Total # of Samples Analyzed AOAC Research Institute Expert Review Panel Use Only 0/132 64/132 132/132 Reference POD s r

s L

s R

P Value

1.0000

-0.01 (-0.04, 0.02)

dLPOD (Candidate vs. Reference) f

dLPOD (Candidate Presumptive vs. Candidate Confirmed) f -0.01 (-0.04, 0.02) a Results include 95% Confidence Intervals, r Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation 2 e P Value = Homogeneity test of laboratory PODs; f A confidence interval for dLPOD that does not contain the value 0 indicates a statistical significant difference between 3 the two methods 4

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