AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

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procedure. There were 7 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9

and 13.

Naturally Contaminated raw chicken leg pieces (25 g)

Lot 1

For lot 1 of the raw chicken leg pieces of the MDA 2 - Listeria assay, there were 18 presumptive positives and 18 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 16 observed positives for the reference method. Detailed

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results of the POD analyses are presented in Tables 9 and 13.

Lot 2

For lot 2 of the raw chicken leg pieces of the MDA Listeria - 2 assay, there were 12 presumptive positives and 12 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 14 observed positives for the reference method. Detailed

results of the POD analyses are presented in Tables 9 and 13.

Whole melon

For the low inoculation level of the MDA 2 - Listeria assay, there were 12 presumptive positives and 10 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure, resulting in 2 false positives. There were 8 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses

are presented in Tables 9 and 13.

Vanilla ice cream (25 g)

AOAC Research Institute Expert Review Pan l Use Only

For the low inoculation level of the MDA 2 - Listeria assay, there were 7 presumptive positives and 7 confirmed positives following the AOAC 993.12 reference method confirmation procedure. There were 7 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

Queso fresco (25 g)

For the low inoculation level of the MDA 2 - Listeria assay, there were 11 presumptive positives and 11 confirmed positives following the AOAC 993.12 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12