AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

3M Molecular Detection Assay 2 Half Fraser broth 24h at 37°C

Inoculation level (cfu/225ml)

Genus

Species

Reference Origin

Confirmation 100µl

MDA 2 - Listeria spp

ALOA

PALCA

Listeria ivanovii Listeria ivanovii Listeria ivanovii Listeria ivanovii Listeria ivanovii Listeria ivanovii

32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50

Ad 662 BR11 Ad 1289 Ad 1290 Ad 1291 Ad 1288

Environment (dairy industry)

20 29 30 17 18 80 14 29 36 28 28 36 44 26 24 46 33 8

+ + + + + + + + + + + + + + + + + + +

H+ H+ H+ H+ H+ H+

+ + + + + + + + + + + + + + + + +

Environment (fish) Raw milk cheese

Milk powder

Poultry

Sheep milk

Listeria ivanovii subsp. londoniensis Listeria seeligeri Listeria seeligeri Listeria seeligeri Listeria seeligeri Listeria seeligeri Listeria seeligeri Listeria welshimeri Listeria welshimeri Listeria welshimeri Listeria welshimeri Listeria welshimeri Listeria seeligeri

CIP103466 Unknown

H- H- H- H- H- H- H- H- H- H- H- H- st

Ad 649 Ad 651 Ad 652 Ad 674

Cheese

Environment

Environment (dairy industry)

Soft cheese (Munster)

240

H-

BR1 BR18

Trout

Environment (fish)

CIP100100 Unknown

1col

Ad1276 Ad1235 191424 Ad 1175 Ad 650

Environment (Slaughterhouse)

Beef meat

Poultry

Ready-to-eat-food

Poultry

1 All the strains are wild strains isolated in Adria Developpement, Quimper, France. 2 Ad = Adria 3 A= Adria 4 BR = ANSES Lab strain 5 CIP = collection of Pasteur Institute 6 H+/- = Presence/absence Phospho-Lipolysis halo 7 St = plate without colonies 8 ANSES = French Agency for Food, Environmental, and Occupational Health and Safety, Cedex, France 9 Listeria strains #21 and #22 utilized UHT to assist with growth as ISO 16140 directs.

AOAC R search Institute Expert Review Panel Use Only

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