AOAC-RI ERP Micro June 2016
OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only
Table 4a: Independent Study - Matrixes and Inoculating Organisms
1
MDA 2 Testing Time Points
Inoculating Organism (Culture Condition) L. monocytogenes ATCC 1 19116 (heat-stressed)
# of Replicates
Reference Method (Enrichment)
Matrix
Target Inoculum Level
0 CFU/Test Portion
5
USDA/FSIS MLG 8.09 (UVM)
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
Deli Turkey
24 hours
5
Lot 1 & Lot 2
20 & 20
Raw Chicken Leg Pieces
Naturally Contaminated
USDA/FSIS MLG 8.09 (UVM)
28 hours
0 CFU/Test Portion
5
L. monocytogenes FSL 3 J1-049
FDA/BAM Chapter 10 (BLEB)
26 hours
Whole Melon
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5 5 5 5
L. monocytogenes ATCC 1 19114
Vanilla Ice Cream
AOAC 993.12 (Selective Enrichment)
20
24 hours
0 CFU/Test Portion
L. monocytogenes CWD 2 1554 (heat-stressed) L. monocytogenes ATCC 1 19112 & L. welshimeri ATCC 1 35897 L. monocytogenes ATCC 1 7644 (heat-stressed) L. monocytogenes ATCC1 19118 & 10x Enterococcus faecium ATCC 19434
AOAC 993.12 (Selective Enrichment)
Queso Fresco
24 hours
0.2-2 CFU/Test Portion 2-5 CFU/Test Portion
20
5 5
0 CFU/Test Portion
0.2-2 CFU/Test Portion
20
4% Milk Fat Cottage Cheese
AOAC 993.12 (Selective Enrichment)
24 hours
2-5 CFU/Test Portion
5
0 CFU/Test Portion
5
USDA/FSIS MLG 8.09 (UVM)
Beef Hot Dogs
0.2-2 CFU/Test Portion
20
24 hours
2-5 CFU/Test Portion 0 CFU/1” x 1” (5 cm2) ~50 CFU/1” x 1” (5 cm2) 0 CFU/4” x 4” (100 cm2) AOAC Research Institute Expert Review Panel Use Only 5 5 24 and 26 hours USDA/FSIS MLG 8.09 (UVM) 20 5 0 CFU/4” x 4” (100 cm2) 5
Stainless Steel
L. monocytogenes ATCC1 19117
Sealed Concrete
~50 CFU/4” x 4” (100 cm2) ~100 CFU/4” x 4” (100 cm2) 0 CFU/1” x 1” (5 cm2) ~50 CFU/1” x 1” (5 cm2) ~100 CFU/1” x 1” (5 cm2)
24 and 26 hours
USDA/FSIS MLG 8.09 (UVM)
20
5
L. monocytogenes ATCC1 51782 & L. seeligeri ATCC1 35967 &
5
24 and 26 hours
USDA/FSIS MLG 8.09 (UVM)
20
Plastic
5
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