AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

Table 4a: Independent Study - Matrixes and Inoculating Organisms

1

MDA 2 Testing Time Points

Inoculating Organism (Culture Condition) L. monocytogenes ATCC 1 19116 (heat-stressed)

# of Replicates

Reference Method (Enrichment)

Matrix

Target Inoculum Level

0 CFU/Test Portion

5

USDA/FSIS MLG 8.09 (UVM)

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

Deli Turkey

24 hours

5

Lot 1 & Lot 2

20 & 20

Raw Chicken Leg Pieces

Naturally Contaminated

USDA/FSIS MLG 8.09 (UVM)

28 hours

0 CFU/Test Portion

5

L. monocytogenes FSL 3 J1-049

FDA/BAM Chapter 10 (BLEB)

26 hours

Whole Melon

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5 5 5

L. monocytogenes ATCC 1 19114

Vanilla Ice Cream

AOAC 993.12 (Selective Enrichment)

20

24 hours

0 CFU/Test Portion

L. monocytogenes CWD 2 1554 (heat-stressed) L. monocytogenes ATCC 1 19112 & L. welshimeri ATCC 1 35897 L. monocytogenes ATCC 1 7644 (heat-stressed) L. monocytogenes ATCC1 19118 & 10x Enterococcus faecium ATCC 19434

AOAC 993.12 (Selective Enrichment)

Queso Fresco

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5

0 CFU/Test Portion

0.2-2 CFU/Test Portion

20

4% Milk Fat Cottage Cheese

AOAC 993.12 (Selective Enrichment)

24 hours

2-5 CFU/Test Portion

5

0 CFU/Test Portion

5

USDA/FSIS MLG 8.09 (UVM)

Beef Hot Dogs

0.2-2 CFU/Test Portion

20

24 hours

2-5 CFU/Test Portion 0 CFU/1” x 1” (5 cm2) ~50 CFU/1” x 1” (5 cm2) 0 CFU/4” x 4” (100 cm2) AOAC Research Institute Expert Review Panel Use Only 5 5 24 and 26 hours USDA/FSIS MLG 8.09 (UVM) 20 5 0 CFU/4” x 4” (100 cm2) 5

Stainless Steel

L. monocytogenes ATCC1 19117

Sealed Concrete

~50 CFU/4” x 4” (100 cm2) ~100 CFU/4” x 4” (100 cm2) 0 CFU/1” x 1” (5 cm2) ~50 CFU/1” x 1” (5 cm2) ~100 CFU/1” x 1” (5 cm2)

24 and 26 hours

USDA/FSIS MLG 8.09 (UVM)

20

5

L. monocytogenes ATCC1 51782 & L. seeligeri ATCC1 35967 &

5

24 and 26 hours

USDA/FSIS MLG 8.09 (UVM)

20

Plastic

5