AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

Table 4b: Internal Study - Matrixes and Inoculating Organisms

1

Inoculating Organism (Culture Condition)

MDA 2 Testing Time Points

# of Replicates

Reference Method (Enrichment) USDA/FSIS MLG 8.09 (UVM) USDA/FSIS MLG 8.09 (UVM)

Matrix

Target Inoculum Level

0 CFU/Test Portion

5

Raw chicken 2 leg pieces

L. ivanovii Ad 1 1291

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

28 hours

5 5

0 CFU/Test Portion

L. ivanovii Ad 1291

Raw chicken fillet 3

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

28 hours

5 5

0 CFU/Test Portion

FDA/BAM Chapter 10 (BLEB)

Bagged raw 2 spinach

Listeria seeligeri Ad 1754

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5

0 CFU/Test Portion

ISO 11290-1/A1 (Half Fraser/Demi Fraser)

Listeria seeligeri Ad 1754

Bagged raw 2 spinach

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion 0 CFU/Test Portion 0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5 5 5 5

FDA/BAM Chapter 10 (BLEB)

Cold smoked 2 salmon

Listeria innocua Ad 1674

20

24 hours

0 CFU/Test Portion

ISO 11290-1/A1 (Half Fraser/Demi Fraser)

Cold smoked 2 salmon

Listeria innocua Ad 1674

24 hours

0.2-2 CFU/Test Portion 2-5 CFU/Test Portion

20

5

2 3 4

1 Ad – Adria Developpement Culture Collection 2 Tested using Demi Fraser brand (with FAC) X 3 Tested using Demi Fraser brand (with FAC) Z

AOAC Research Institute Expert Review Panel Use Only