AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

Table 5a: Independent Study - Aerobic Plate Count and Background Results (Prior to Inoculation)

Background Listeria (# positive/total tested)

Matrix (Test Portion)

APC 1 (CFU/g)

Deli Turkey (125 g)

1.5 x 10 3

0/5 (2)

Naturally Contaminated Raw Chicken Leg Pieces (25 g)

2.8 x 10 5

4/5 (2)

1.0 x 10 2

0/5 (3)

Whole Melons

Vanilla Ice Cream (25 g) Queso Fresco (25 g) 4% Milk Fat Cottage Cheese (25 g) Beef Hot Dogs (25 g) Beef Hot Dogs 5 (25 g)

8.0 x 10 1

0/5 (4)

3.8 x 10 5

0/5 (4)

1.7 x 10 4

0/5 (4)

1.6 x 10 2

0/5 (2)

6.0 x 10 3

0/5 (2)

1 APC conducted in accordance with FDA/BAM Chapter 3 2 Listeria species screen conducted following the USDA/FSIS MLG 8.09 guidelines 3 Listeria species screen conducted following the FDA/BAM Chapter 10 guidelines 4 Listeria species screen conducted following the AOAC 993.12 guidelines 5 Matrix used to conduct Robustness and Lot-to-Lot testing

Table 5b: Internal Study - Aerobic Plate Count and Background Results (Prior to Inoculation)

Background Listeria (# positive/total tested)

Matrix (Test Portion)

APC 1 (CFU/g)

Raw Chicken Leg Pieces (25 g) Raw chicken fillet (25 g) Bagged Raw Spinach (25 g) Cold Smoked Salmon (25 g)

4.0 x 10 5

0/5 (2)

1.4 x 10 5 AOAC Research Institute Expert Review Panel Use Only 0/5 (2) 9.3 x 10 6 0/5 (3,4) 2.0 x 10 2 0/5 (3,4)

1 APC conducted in accordance with FDA/BAM Chapter 3 2 Listeria species screen conducted following the USDA/FSIS MLG 8.09 guidelines 3 Listeria species screen conducted following the FDA/BAM Chapter 10 guidelines 4 Listeria species screen conducted following the ISO 11290-1/1A guidelines