AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

Table 7: Inoculum Summary Table for Whole Melons and Environmental Surfaces Matrix Whole Melons Inoculating Organism Listeria monocytogenes FSL J1-049 Low-Inoculum Level CFU a /Melon b 7 High-Inoculum Level CFU a /Test Melon b 55 Matrix Stainless Steel Inoculating Organism Listeria monocytogenes ATCC 19118

Enterococcus faecium ATCC 19434

Low-Inoculum Level CFU a /Test Area c High-Inoculum Level CFU a /Test Area c Inoculating Organism Low-Inoculum Level CFU a /Test Area c High-Inoculum Level CFU a /Test Area c Inoculating Organism Low-Inoculum Level CFU a /Test Area d High-Inoculum Level CFU a /Test Area d Matrix Matrix

42

530

400

4000

Sealed Concrete

Listeria monocytogenes ATCC 19117

35

360

Plastic

Listeria monocytogenes ATCC 51782

Listeria seeligeri ATCC 35967

Enterococcus faecalis ATCC 29212

42

64

540

420

590

3600

a CFU: aliquots of the inocula were plated in triplicate onto TSA and averaged b Test Area: Whole Melon

c Test Area: 4” x 4” Surface Area d Test Area: 1” x 1” Surface Area

AOAC Research Institute Expert Review Panel Use Only