AOAC-RI ERP Micro June 2016

OMAMAN-30 A/ Collaborative Study Manuscript OMA ERP June 2016 ERP Use Only

(m) Empty Reagent Tube Rack - Available from 3M Food Safety (St. Paul, MN 55144-

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1000, USA).

(n) Demi Fraser Broth - Available from 3M Food Safety (St. Paul, MN 55144-1000,

USA).

(o) Ferric Ammonium Citrate (FAC), ACS grade, 5% sterilized - Available from MP

Biomedicals™ or equivalent.

(p) Disposable pipette – capable of 20 µL (q) Multi - channel (8-channel) pipette - capable of 20 µL (r) Sterile filter tip pipette tips - capable of 20 µL (s) Filter Stomacher® bags – Seward or equivalent .

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(t) Stomacher ®– Seward or equivalent. (u) Thermometer – calibrated range to include 100 ± 1 o C (v) Dry block heater unit– capable of maintaining 100 ± 1 o C (w) Incubators. – Capable of maintaining 37 ± 1°C or 41.5 ± 1°C. (x) Freezer – capable of maintaining -10 to -20 o C, for storing the 3M Molecular

Detection Chill Block Tray

(y) Refrigerator – capable of maintaining 2-8 o C, for storing the 3M Molecular Detection

Assay components

(z) Computer – compatible with the 3M ™ Molecular Detection Instrument 3M™ Enviroswab, -- (hydrated with Letheen) Available from 3M Food (aa)

Safety(Australia)

(bb) 3M™ Hydrated Sponge Stick with 10 mL of D/E – Available from 3M Food

Safety(St. Paul, MN 55144-1000, USA)

C. General Instructions

(a) Store the 3M Molecular Detection Assay 2 - Listeria monocytogenes at 2-8°C. Do not freeze. Keep kit away from light during storage. After opening the kit, check that the foil pouch is undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes should always be stored in the re-sealable pouch with the desiccant inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2- 8°C for no longer than 60 days. Do not use 3M Molecular Detection Assay 2 - Listeria monocytogenes past the expiration date. AOAC Research Institute Expert Review Panel Use Only (b) Follow all instructions carefully. Failure to do so may lead to inaccurate results. Safety Precautions The 3M MDA2 – Listeria monocytogenes is intended for use in a laboratory environment by professionals trained in laboratory techniques. 3M has not documented the use of this product in industries other than the food and beverage industries. For example, 3M has not documented this product for testing drinking water, pharmaceutical, cosmetics, clinical or veterinary samples. The 3M MDA2 – Listeria monocytogenes has not been evaluated with all possible food products, food

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processes, testing protocols or with all possible strains of bacteria.

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