AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

Lisa Monteroso Study Director 3M Food Safety Department 3M Center, Building 260-6B-01 St. Paul, MN 55144 Phone: 651-736-2913 Fax: 651-733-5819 Email: lmonteroso@mmm.com

Patrick Bird & Erin Crowley Co-Study Directors Q Laboratories, Inc. 1400 Harrison Avenue Cincinnati, Ohio 45214 Phone : 513-471-1300 Fax : 513-471-5600 Email: pbird@qlaboratories.com ecrowley@qlaboratories.co

2.0 Collaborators A total of 15-16 collaborators will be solicited to participate in this study. A minimum of 10 laboratories, with acceptable data, will be needed for the successful completion of this study. Laboratories currently using the 3M MDS or those who will be thoroughly trained by a 3M representative, with a clear understanding of the reference methods, will act as collaborators for this study. A sample letter to collaborators can be found in Appendix 9.1. This letter will be sent to each collaborator prior to the start of the study. 3.0 Collaborative Study Design 3.1 This collaborative study is designed according to the AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces (2012). 3.2 In this study, two food types will be analyzed: 3.2.1 Deli turkey (125g) – 3M MDA 2 - Listeria and 3M MDA 2 - Listeria monocytogenes compared to USDA/FSIS MLG 8.09 1 3.2.2 Raw chicken breast (25g) – 3M MDA 2 - Listeria and 3M MDA 2 - Listeria monocytogenes compared to USDA/FSIS MLG 8.09 3.3 Collaborators will receive test portions for each matrix: 3.3.1 Raw chicken breast – 36 x 60g samples. There will be 12 uninoculated control, 12 low level (target 0.2 – 2.0 cfu/test portion), and 12 high level (target 2 – 5 cfu/test portion) samples. Each sample will be divided into two separate 25g test portions: one will be tested using the 3M MDA 2test kits ( Listeria and Listeria monocytogenes ) and the other will be tested by the reference method. 3.3.2 Deli turkey – 72 x 125g samples. There will be 24 uninoculated control, AOAC Research Institute Expert Review Panel Use Only

24 low level (target 0.2 – 2.0 cfu/test portion), and 24 high level (target 2 – 5 cfu/test portion) samples. Thirty-six 125g test portions (12 at each level)

1 http://www.fsis.usda.gov/wps/wcm/connect/1710bee8-76b9-4e6c-92fc-fdc290dbfa92/MLG-8.pdf?MOD=AJPERES

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