AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

will be tested using the 3M MDA 2 test kits ( Listeria and Listeria monocytogenes ) and thirty-six 125g test portions (12 at each level) will be tested by the reference method. 3.3.3 Each laboratory will test all test portions. Each food will be inoculated with a different Listeria monocytogenes strain as listed in Table 1 and will be randomized and blind-coded for analysis. 3.4 Study Schedule 3.4.1 One matrix will be tested each week of the study. All data should be sent to the Study Director by the 4 th week of study. 3.4.2 The contract laboratory will prepare the test portions and ship the test portions refrigerated via overnight courier to the collaborators to arrive on a Friday. Test portion processing and analysis will begin on Monday. Data forms will be completed and faxed or emailed back to the co-Study Director by the following Friday.

Table 1. Overview of Study Design

Target L. monocytogenes Levels

Inoculating organism

Test Portion Codes

Reference Method

MATRIX

Raw chicken breast

0 cfu/test portion

Random number between 200-299 Random number between 300-399

USDA/FSIS MLG 8.09 Rev. 09 (5/1/2013)

L. monocytogenes TBD

25g – 3M MDA2 25g – USDA/FSIS MLG Deli turkey 125g – 3M MDA2 125g – USDA/FSIS MLG

0.2-2 cfu/test portion 2-5 cfu/test portion

0 cfu/test portion

L. monocytogenes TBD USDA/FSIS MLG 8.09 Rev. 09 (5/1/2013) 0.2-2 cfu/test portion 2-5 cfu/test portion AOAC Research Institute Expert Review Panel Use Only

5/4/16