AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

4.2.3 Extra caps. – 96 (12 strips of 8 caps) 4.2.4 Reagent Control (RC). – 16 (2 pouches of 8) 4.2.5 Quick Start Guide

Additional Supplies and Reagents 4.3.1 3M Molecular Detection System (instrument), power supply, cord, USB cable and software 4.3.2 3M Molecular Detection Speed Loader Tray 4.3.3 3M Molecular Detection Chill Block Tray and Chill block insert 4.3.4 3M Molecular Detection Heat Block Insert 4.3.5 3M Molecular Detection Cap/Decap Tool [Reagent] 4.3.6 3M Molecular Detection Cap/Decap Tool [Lysis]

4.3

4.3.7 Empty lysis tube rack 4.3.8 Empty reagent tube rack 4.3.9 Dey-Engley (D/E) Neutralizing Broth 4.3.10 Demi-Fraser broth

Additional media 4.4.1 Oxford Agar (OX) 4.4.2 Sheep blood agar (SBA) Apparatus 4.5.1 Pipettes . – capable of 20µL

4.4

4.5

4.5.2 Multi-channel pipette . – capable of 20µL 4.5.3 Sterile pipette tips. – capable of 20µL 4.5.4 Stomacher® . – Seward or equivalent 4.5.5 Filter Stomacher® bags . - Seward or equivalent 4.5.6 Thermometer . – calibrated range to include 100 ± 1°C range 4.5.7 Incubators . – capable of maintaining 37 ± 1°C 4.5.8 Dry double block heater unit. – capable of maintaining 100 ± 1°C; OR a water bath capable of maintaining 100 ± 1°C 4.5.9 Freezer . – capable of maintaining -10 to -20°C, for storing the 3M Molecular Detection Chill Block Tray 4.5.10 Refrigerator . – capable of maintaining 2-8°C, for storing the 3M MDA 4.5.11 Computer . – compatible with the 3M Molecular Detection System (instrument) AOAC Rese rch Institute Expert Review Panel Use Only

Determination 4.6.1 Enrichment

4.6

4.6.1.1 Deli turkey - aseptically add the 125g test portion to 1125 mL Demi Fraser broth. 4.6.1.1.1 Homogenize thoroughly in a filter bag for 2 minutes ± 30 seconds 4.6.1.1.2 Incubate 24-30 hr at 37 ± 1°C.

5/4/16