AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

4.6.14 Confirmation

All positive 3M MDA2 - Listeria results must be confirmed. Confirmation should be performed using the primary enrichment, carried through as described in the reference method starting with the secondary transfer and selective isolation. Confirmation should be initiated within 72 hrs. Isolate on a selective agar and identify 1 to 5 typical colonies according to the conventional tests described in the AOAC method.

5.0

3M MDA 2 - Listeria monocytogenes 5.1 Applicability

For the detection of Listeria monocytogenes from: 5.1.1 Beef hot dogs (25 g and 125 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.2 Cold smoked salmon (25 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.3 Deli turkey (25 g and 125 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.4 Full fat cottage cheese (25 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.5 Queso fresco (25 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.6 Chocolate milk (25 mL): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.7 Vanilla ice cream (25 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.8 Romaine lettuce (25 g): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.9 Cantaloupe (whole melon): 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.10 Raw chicken leg pieces (25 g): 28-32 hours of enrichment in Demi Fraser broth at 37°C 5.1.11 Stainless steel : sponge in 225 mL with 24-30 hours of enrichment in Demi Fraser broth at 37°C 5.1.12 Concrete : sponge in 100 mL and 225 mL and sponge in 225 mL with24- 30 hours of enrichment in Demi Fraser broth at 37°C 5.1.13 Plastic : Enviroswab in 10 mL 24-30 hours of enrichment in Demi Fraser broth at 37°C Caution : The 3M MDA 2 - Listeria monocytogenes is intended for use in a laboratory environment by professionals trained in laboratory techniques. The user should read, understand and follow all safety information in the instructions for the 3M MDS and the 3M MDA 2 - Listeria monocytogenes and retain the safety instructions for future reference. Follow all instructions carefully. Failure to do so may lead to inaccurate results. AOAC Research Institute Expert Review Panel Use Only

5/4/16