AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

Appendix 8.3a – Study Flow Diagram: Deli Turkey 1 2

Deli Turkey - 125g

3MMDA 2- Listeria & 3MMDA 2- L. monocytogenes

USDA/FSIS MLG 8.09

125g + 1125 mL prewarmed (37±1C ° ) Demi Fraser (no FAC), 24-28 hr at 37±1C °

125g + 1125 mL UVM 23-26 hr at 30±2C °

Presumptive Result Using enriched sample, run 3M MDA Listeria monocytogenes test kit

MDA Confirmation Swab and streak to MOX, 24-28 hr hr at 35±2C °

MDA Confirmation Transfer 0.1 mL to Fraser broth, 24-28 hr hr at 35±2C °

Transfer 0.1 mL to Fraser broth, 24-28 hr hr at 35±2C °

Swab and streak to MOX, 24-28 hr hr at 35±2C °

Swab and streak to MOX, 24-28 hr hr at 35±2C °

Swab and streak to MOX, 24-28 hr hr at 35±2C °

Streak suspect colonies to TSA, 24 hr at 37±1 ° C

Streak suspect colonies to TSA, 24 hr at 37±1 ° C Streak suspect colonies to TSA, 24 hr at 37±1 ° C AOAC Research Institute Expert Review Panel Use Only Streak suspect colonies to TSA, 24 hr at 37±1 ° C

ID at least one colony using VITEK 2 GP or API Listeria

ID at least one colony using VITEK 2 GP or API Listeria

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