AOAC-RI ERP Micro June 2016
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only
3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX
Appendix 8.4
1
Study Materials 2 3 Materials and reagents provided by the study sponsor
4 5
1. 3M Molecular Detection 2 kits 6 2. 3M Molecular Detection System (instrument), power supply, cord, USB cable and 7 software 8 3. 3M Molecular Detection Speed Loader Tray 9 4. 3M Molecular Detection Chill Block Insert 10 5. 3M Molecular Detection Heat Block Insert 11 6. 3M Molecular Detection Cap/Decap Tool [Reagent] 12 7. 3M Molecular Detection Cap/Decap Tool [Lysis] 13 8. Empty lysis tube rack 14 9. Empty reagent tube rack 15 10. Sterile filter-tip pipette tips – capable of 20µL 16 11. Filter Stomacher® bags – Seward or equivalent
17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33
Enrichment 12. UVM
13. Demi Fraser broth with Ferric Ammonium Citrate (FAC)
14. Fraser broth with FAC
Selective agar
15. MOX
Confirmation
16. Blood agar (HL and SBA)
17. Catalase 18. Oxidase
19. Motility supplies
AOAC Research Institute Expert Review Panel Use Only
20. Tryptic Soy Agar with yeast extract 21. VITEK2 GP or API Listeria
5/4/16
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