AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

To reduce the risks associated with environmental contamination: • Follow current industry standards for disposal of contaminated waste.

CAUTION • Do not exceed the recommended temperature setting on heater. 1 • Do not exceed the recommended heating time. 2 • Use an appropriate, calibrated thermometer to verify the 3M™ Molecular Detection Heat 3 Block Insert temperature (e.g., a partial immersion thermometer or digital thermocouple 4 thermometer, not a total immersion thermometer.) The thermometer must be placed in the 5 designated location in the 3M Molecular Detection Heat Block Insert. NOTICE To reduce the risks associated with cross-contamination while preparing the assay: 8 • Use of sterile, aerosol barrier (filtered), molecular biology grade pipette tips is 9 recommended. 10 • Use a new pipette tip for each sample transfer. 11 • Use Good Laboratory Practices to transfer the sample from the enrichment to the lysis tube. 12 To avoid pipettor contamination, the user may choose to add an intermediate transfer step. 13 For example, the user can transfer each enriched sample into a sterile tube. 14 • Use a molecular biology workstation containing germicidal lamp where available. 6 7 • Never open tubes post amplification. 18 • Always dispose of the contaminated tubes by soaking in a 1-5% (v:v in water) household 19 bleach solution for 1 hour and away from the assay preparation area. 20 21 Consult the Safety Data Sheet for additional information and local regulations for disposal. 22 23 If you have questions about specific applications or procedures, please visit our website at 24 www.3M.com/foodsafety or contact your local 3M representative or distributor. 15 16 17 To reduce the risks associated with a false-positive result:

AOAC Research Institute Expert Review Panel Use Only

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LIMITATION OF WARRANTIES / LIMITED REMEDY

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