AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

Detection Assay 2 – Listeria monocytogenes method is 1-5 colony forming units per

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validated test portion size (in Table 3).

Table 3. Enrichment protocols using Demi-Fraser Broth at 37 ± 1 °C according to AOAC Performance Tested SM Certificate #XXXXXX

Enrichment Broth Volume (mL)

Enrichment Time (hr)

Sample Matrix

Sample Size

Beef hot dogs, Queso Fresco, Vanilla Ice Cream, 4% Milk Fat Cottage Cheese, 3% chocolate whole milk, romaine lettuce, bagged raw spinach, cold smoked salmon

25 g

225

24-30

Raw chicken

25 g

475

28-32

Deli turkey

125 g

1125

24-30

Enough volume to allow melon to float

26-30

Cantaloupe

Whole melon

Stainless steel Sealed concrete

1 sponge

225

24-30

1 sponge AOAC Research Institute Expert Review Panel Use Only 100 Plastic 1 swab 10 24-30

Environmental samples:

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P REPARATION OF THE 3M™M OLECULAR D ETECTION S PEED L OADER T RAY 10

1. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™

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Molecular Detection Speed Loader Tray.

2. Rinse the 3M Molecular Detection Speed Loader Tray with water.

3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry. 4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.

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