AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

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R EFERENCES : 2 1. US Food and Drug Administration Bacteriological Analysis Manual. Chapter 10: Detection 3 and Enumeration of Listeria monocytogenes in Foods. Section C-6. April 2011 Version. 4 2. US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08. 5 Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry and Egg 6 Products, and Environmental Samples. Effective Date: 6 Nov 2012. 7 3. ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the 8 Detection and Enumeration of Listeria monocytogenes . Amendment 1, 2004-10-15. 9 4. ISO/IEC 17025. General requirements for the competence of testing and calibration 10 laboratories. 11 5. ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological 12 examination. 13 6. ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for 14 sampling techniques from surfaces using contact plates and swabs. 15 16 Appendix A. Protocol Interruption: Storage and re-testing of heat-treated lysates 17 1. To store a heat-treated lysate, re-cap the lysis tube with a clean cap (see “L YSIS ”, 4.5) 18 2. Store at 4 to 8°C for up to 72 hours. 19 3. Prepare a stored sample for amplification by inverting 2-3 times to mix. 20 4. Decap the tubes. 21 5. Place the mixed lysate tubes on 3M Molecular Detection Heat Block Insert and heat at 100 22 ±1°C for 5 ±1 minutes. 23 6. Remove the rack of LS tubes from the heating block and allow to cool in the 3M Molecular 24 Detection Chill Block Insert at least 5 minutes and a maximum of 10 minutes. 25 7. Continue the protocol at the ‘Amplification’ section detailed above.

AOAC Research Institute Expert Review Panel Use Only

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EXPLANATION OF PRODUCT LABEL SYMBOLS

Caution or Warning, see product instructions

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