AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

amplification. • Dispose of enriched samples according to current industry standards.

To reduce the risks associated with cross-contamination while preparing the assay: • Always wear gloves (to protect the user and prevent introduction of nucleases). To reduce the risks associated with environmental contamination: • Follow current industry standards for disposal of contaminated waste. CAUTION • Do not exceed the recommended temperature setting on heater. 1 • Do not exceed the recommended heating time. 2 • Use an appropriate, calibrated thermometer to verify the 3M™ Molecular Detection Heat 3 Block Insert temperature (e.g., a partial immersion thermometer or digital thermocouple 4 thermometer, not a total immersion thermometer.) The thermometer must be placed in the 5 designated location in the 3M Molecular Detection Heat Block Insert. NOTICE To reduce the risks associated with cross-contamination while preparing the assay: 8 • Use of sterile, aerosol barrier (filtered), molecular biology grade pipette tips is 9 recommended. 10 • Use a new pipette tip for each sample transfer. 11 • Use Good Laboratory Practices to transfer the sample from the enrichment to the lysis tube. 12 To avoid pipettor contamination, the user may choose to add an intermediate transfer step. 13 For example, the user can transfer each enriched sample into a sterile tube. 14 • Use a molecular biology workstation containing germicidal lamp where available. 6 7

AOAC Research Institute Expert Review Panel Use Only

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To reduce the risks associated with a false-positive result:

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