AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

smoked salmon

Deli Turkey

125 g

1175

24-30

stainless steel, sealed concrete

1 sponge

100 or 225

24-30

Plastic,

1 swab

10

24-30

Environmental samples:

Raw Chicken

25 g

475

28-32

1 P REPARATION OF THE 3M™M OLECULAR D ETECTION S PEED L OADER T RAY 2 5. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™ 3 Molecular Detection Speed Loader Tray. 4 6. Rinse the 3M Molecular Detection Speed Loader Tray with water. 5 7. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry. 6 8. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use. 7 P REPARATION OF THE 3M™M OLECULAR D ETECTION C HILL B LOCK I NSERT 8 Place the 3M™ Molecular Detection Chill Block directly on the laboratory bench; (the 3M™ 9 Molecular Detection Chill Block Tray is not used). Use the chill block at ambient laboratory 10 temperature (20-25°C). 11 12 P REPARATION OF THE 3M™M OLECULAR D ETECTION H EAT B LOCK I NSERT 13 Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on 14 the dry block heater unit and set the temperature to allow the 3M Molecular Detection Heat 15 Block Insert to reach and maintain a temperature of 100 ±1°C. 16 17 NOTE: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular 18 Detection Heat Block Insert to reach temperature. Using an appropriate, calibrated thermometer 19 (e.g., a partial immersion thermometer or digital thermocouple thermometer, not a total 20 immersion thermometer) placed in the designated location, verify that the 3M Molecular 21 Detection Heat Block Insert is at 100 ±1°C. AOAC Research Institute Expert Review Panel Use Only

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