AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

Presumptive positive samples should be confirmed as per the laboratory standard operating 1 procedures or by following the appropriate reference method confirmation (1, 2, 3) , beginning with 2 transfer from the primary enrichment to secondary enrichment broth (if applicable), followed by 3 subsequent plating and confirmation of isolates using appropriate biochemical and serological 4 methods. 5 NOTE: Even a negative sample will not give a zero reading as the system and 3M Molecular 6 Detection Assay 2 - Listeria monocytogenes amplification reagents have a “background” relative 7 light unit (RLU). 8 9 In the rare event of any unusual light output, the algorithm labels this as “Inspect.” 3M 10 recommends the user to repeat the assay for any Inspect samples. If the result continues to be 11 Inspect, proceed to confirmation test using your preferred method or as specified by local 12 regulations 13 14 If you have questions about specific applications or procedures, please visit our website at 15 www.3M.com/foodsafety or contact your local 3M representative or distributor. 18 7. US Food and Drug Administration Bacteriological Analysis Manual. Chapter 10: Detection 19 and Enumeration of Listeria monocytogenes in Foods. Section C-6. April 2011 Version. 20 8. US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08. 21 Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry and Egg 22 Products, and Environmental Samples. Effective Date: 6 Nov 2012. 23 9. ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the 24 Detection and Enumeration of Listeria monocytogenes . Amendment 1, 2004-10-15. 25 10. ISO/IEC 17025. General requirements for the competence of testing and calibration 26 laboratories. 27 11. ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological 28 examination. 29 12. ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for 30 sampling techniques from surfaces using contact plates and swabs. 31 32 Appendix A. Protocol Interruption: Storage and re-testing of heat-treated lysates 33 8. To store a heat-treated lysate, re-cap the lysis tube with a clean cap (see “L YSIS ”, 4.5) 34 AOAC Research Institute Expert Review Panel Use Only 16 17 R EFERENCES :

54