MCPD-02 Reviewer Forms (July 19, 2018)

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Reviewer 7

Reproducibility (RSD R

): Not specifically listed in the validation data as RSD R

, but multi-day, multi-

analyst data was provided with RSDs <10% listed across analytes.

• System suitability: results for reference materials were provided; however, there was no specific listing of check standard results at low point and midpoint of ranges. Blank sample checks were included (cold pressed oils). Variability of fat extraction related to matrix composition not thoroughly addressed. Detailed information on the sample compositions and fat content was not provided. Adult nutritional samples not included. Addressed in revised method. Recommendation: Very concerned about proceeding with a method with single point calibration based on internal standards as the only option. Although I realize that the AOCS reference method uses this same calibration procedure and that the method is likely very suitable for routine process monitoring for a specific matrix type, I feel that there is a risk that appropriate controls will not be put in place at all performing laboratories once issued. I would rather see an option to use an external standard calibration with internal standard correction. Method has been shown to work well in our laboratory with external standard calibration curve utilized, so I am OK with moving forward if panel is OK with moving forward with single point calibration as the only listed means of calibration.

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