MCPD-02 Reviewer Forms (July 19, 2018)

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Reviewer 2 Expert Review Panel for Infant Formula and Adult Nutrition (MCPD)

Evaluation of Method: MCPD-02

Title: 2-MCPD, 3-MCPD and glycidol in infant and adult/pediatric nutritional formula

Author: Jan Huhlman

Reviewer Name:

Summary of Method: Ester bound forms: Fat extraction Mild catalysed transesterification of 2- & 3-MCPDE and GE to free form Glycidol conversion to 3-MBPD Derivatisation with PBA Analysis by GC-MS

Free forms: Extraction Derivatisation with PBA Analysis by GC-MS

Method Scope/Applicability: Infant formula and adult/pediatric nutritional formula, solid (powdered) and liquid. General comments about the method: The method consists of a fat extraction followed by the AOCS Cd 29 c-13 = ISO 18363-1:2017 Method Clarity: The description of the different steps in the form of a text makes it hard to follow, especially when it should be applied in a lab.

Method Safety Concerns:

Pros/Strengths: • “Easier” fat extraction than in the standard method but still effective • No risk of overestimation of GE

Cons/Weaknesses • Use of GC/MS (and not GC-MS/MS) • One-point calibration • Need to apply a transformation factor (3-MCPD to glycidol) which is to be empirically determined for each batch

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