Genomics Application Notes

TABLE OF CONTENTS

Genomics Application Notes Title

Page

˜ ˜ Biomek Automated NGS Library Preparation Methods...................................................................................................................................................... p.1

NGS Applications

˜ ˜ Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FX P Automated Liquid Handler ............................................................................................................................................. p.3 ˜ ˜ High-Throughput TruSeq® Stranded Total RNA Library Construction from FFPE Samples on the Biomek FX P Dual-Arm Multi-96 and Span-8 Workstation.......................................................................................................................................................................... p.9 ˜ ˜ High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FX P Dual-Arm Multi-96 and Span-8 Workstation ......................................................................................................................................................................................................................... p.15

˜ ˜ High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FX P ..................................................................................... p.21

˜ ˜ Automation of the Illumina TruSeq DNA PCR-Free Sample Preparation Kit® on the BiomekFX P Automated Liquid Handler.... p.29

˜ ˜ Automated Directional RNASeq Library Preparation using the Biomek FX P Liquid Handler....................................................................... p.35

˜ ˜ Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FX P HLA SP Liquid Handler........................................................ p.39 Genomic SPRI Nucleic Acid Reagents

˜ ˜ Nucleic acid extraction and purification kits - High recovery of nucleic acids with superior yield and purity.................................... p.47

˜ ˜ Solid Phase Reverse Immobilization (SPRI) Bead Technology for Micro RNA Clean Up using the Agencourt RNAClean XP Kit......................................................................................................................................................................................................... p.53

Agencourt® RNAClean™ XP - Supplemental Protocol for Micro RNA Clean Up using Agencourt® RNAClean® XP.................. p.57

˜ ˜ Micro RNA and Total RNA Purification from Tissues using the Agencourt RNAdvance Tissue Kit........................................................... p.59

Agencourt® RNAdvance™ Tissue Kit - Supplemental Protocol for Micro RNA and Total RNA Isolation from Tissue.............. p.63

˜ ˜ MicroRNA and Total RNA Purification from Blood Stabilized in PAXgene Blood RNA Tubes using the Agencourt RNAdvance Blood Kit ............................................................................................................................................................................................. p.67 Agencourt RNAdvance Blood Kit - Supplemental Protocol for Micro RNA and Total RNA Isolation from PAXgene Preserved Blood................................................................................................................................................................................................... p.70

˜ ˜ MicroRNA Extraction from Exosomes using Beckman’s Agencourt RNAdvance Cell v2 Kits..................................................................... p.74

Agencourt® RNAdvance® CELL v2 Kit - Supplemental Protocol for miRNA and total RNA Isolation from Exosomes.......... p.77

˜ ˜ Automation of micro RNA and Total RNA Purification from Tissues Using the Agencourt RNAdvance Tissue Kits and Biomek Span-8 Liquid Handler..................................................................................................................................................................................................... p.81 ˜ ˜ Automation of Micro RNA and Total RNA Purification from Formalinfixed paraffin-embedded tissues (FFPE) using the Agencourt FormaPure Kit and Biomek NX P Span 8 Laboratory Automation Workstation...................................................... p.85 ˜ ˜ MicroRNA Extraction from Laser Capture Micro- Dissected (LCM) FFPE Tissue Using Beckman Coulter’s Agencourt FormaPure Kits .................................................................................................................................................................................................................................. p.89 ˜ ˜ Highly efficient microRNA and total RNA purification from Formalin-fixed paraffin-embedded tissues (FFPE) using Agencourt FormaPure Kit............................................................................................................................................................................................................... p.91

˜ ˜ PCR Reaction Setup Application - Technical Information Bulletin............................................................................................................................ p.95

˜ ˜ PCR Reaction Setup and AMPure XP Application - Technical Information Bulletin......................................................................................... p.99

˜ ˜ Fast, Reliable Automated PCR Reaction Setup using the Biomek4000 Laboratory Automation Workstation............................... p.103

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