Microsoft Word - 3M Petrifilm REC Plate OMA Manuscript 09121

ERP Methods Book

111

Inoculating Organisms 

Inoculum and stabilizing  conditions 

Matrix 

Non‐ E. coli  coliform Enterobacter cloacae   NBRC 13535  Enterobacter gergoviae   QL 123009‐1  Enterobacter gergoviae   ATCC 33028 

E. coli 

2 Weeks at 20‐25°C  Lyophilized culture  16‐24 h at 20‐25°C  Unstressed culture  16‐24 h at 20‐25°C  Unstressed culture 

Soybean Meal 

E. coli 3M REC11 

Stainless Steel 

E. coli 3M REC1 

Sealed Concrete 

E. coli QL 16160‐10 

a ATCC: American Type Culture Collection, Manassas, VA.  b QL: Q Laboratories Culture Collection, Cincinnati, OH.  c 3M: 3M Food Safety, St. Paul, MN.  d Not applicable.  e NBRC: NITE Biological Resource Center, Tokyo, Japan. 

Table 11: Inoculum heat stress results Matrix 

Inoculating Organism  Klebsiella oxytoca ATCC a 51817 

Agar  TSA b VRB c

CFU/g 

Percent Injury 

6.4 x 10 9  2.2 x 10 9  3.5 x 10 9 8.8 x 10 8 7.2 x 10 9 3.0 x 10 9 4.6 x 10 9 1.5 x 10 9 5.2 x 10 9 1.5 x 10 9 2.4 x 10 9 7.8 x 10 9

65.63% 

Pasteurized  Whole Milk 

TSA

74.86% 

E. coli QL d 16154‐3 

VRB with MUG e

TSA VRB TSA TSA VRB TSA

Klebsiella pneumoniae  QL 11007‐7 

58.33% 

Butter 

67.39% 

E. coli QL 16160‐18 

VRB with MUG

Klebsiella oxytoca ATCC  700324 

71.15% 

Pasteurized  Carrot Juice 

69.17% 

E. coli QL 16160‐9 

VRB with MUG

a ATCC: American Type Culture Collection, Manassas, VA. b TSA: Trypticase Soy Agar.  c VRB: Violet Red Bile Agar.  d QL: Q Laboratories Culture Collection, Cincinnati, OH.  e VRB with MUG: Violet Red Bile Agar with 4‐methyl‐umbelliferyl‐β‐D‐glucuronide.

Page 45 of 61

December 6, 2018