Microsoft Word - 3M Petrifilm REC Plate OMA Manuscript 09121

ERP Methods Book

124

3M Petrifilm Rapid E. coli /Coliform  Count Plate 

ISO 4832:2006 

95% CI g

Cont.  level a

Matrix 

Mean b

s r

RSD r

Mean 

s r

RSD r

Mean diff. f

LCL h

UCL i

d

c

Ready‐ made‐ sandwiches

High 

4.852 

0.077 

1.587 

4.868 

0.076 

1.561 

‐0.016 

‐0.055 

0.023 

Low  Med  High  Low  Med  High  Low  Med  High  Low  Med  High  Low  Med  High 

1.877  2.887  4.852  1.877  2.887  4.934  1.874  2.900  4.852  2.563  3.631  4.303  1.831  2.877  4.801 

0.067  0.076  0.077  0.067  0.076  0.028  0.089  0.055  0.077  0.226  0.146  0.107  0.053  0.067  0.077 

3.570  2.632  1.587  3.570  2.632  0.567  4.749  1.897  1.587  8.818  4.021  2.487  2.895  2.329  1.604 

1.891  2.909  4.853  1.901  2.902  4.908  1.864  2.909  4.881  2.717  3.626  4.477  1.863  2.894  4.829 

0.046  0.064  0.084  0.055  0.067  0.049  0.080  0.053  0.077  0.063  0.079  0.097  0.088  0.062  0.060 

2.433  2.200  1.731  2.893  2.309  0.998  4.292  1.822  1.578  2.319  2.179  2.167  4.724  2.142  1.242 

‐0.013  ‐0.022  ‐0.001  ‐0.024  ‐0.015  0.025  0.010  ‐0.017  ‐0.029  ‐0.154  0.005  ‐0.174  ‐0.032  ‐0.017  ‐0.029 

‐0.076  ‐0.052  ‐0.021  ‐0.089  ‐0.032  ‐0.012  ‐0.043  ‐0.026  ‐0.084  ‐0.414  ‐0.113  ‐0.303  ‐0.154  ‐0.084  ‐0.071 

0.050  0.008  0.018  0.042  0.001  0.063  0.063  ‐0.009  0.025  0.106  0.124  ‐0.044  0.090  0.049 

Raw veg  with  dressing

Chicken feed

Soybean  meal

Stainless  steel

Sealed  concrete 

0.014  a All matrices are artificially contaminated. An uncontaminated level was not tested, as per ISO 16140‐2, Microbiology of the food chain — Method validation —  1 Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method. 2 b Mean of five replicate portions, plated in duplicate, after logarithmic transformation: Log 10 [CFU/g + (0.1)f].   3 c Repeatability standard deviation.  4 d Relative standard deviation for repeatability. Reported as a percentage.  5 e ISO 4832:2006, Microbiology of food and animal feeding stuffs ‐‐ Horizontal methods for the enumeration of coliforms – Colony‐ count technique.  6 f Mean difference between the candidate and reference methods.  7 g Confidence interval based on paired statistical analysis.   8 h 95% Lower confidence limit for difference of means.  9 i 95% Upper confidence limit for difference of means. 

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December 6, 2018