Microsoft Word - 3M Petrifilm REC Plate OMA Manuscript 09121

ERP Methods Book

78

No confirmation is required, however, if necessary colonies may be isolated for further  1

identification. Lift the top film and pick the colony from the gel. Test using appropriate  2

3

reference method procedures. 

4

5

Validation Study 

6

This validation study was conducted under the AOAC Research Institute Performance Tested  7

Method SM  (PTM) program and the AOAC INTERNATIONAL Methods Committee Guidelines for  8

Validation of Microbiological Methods for Food and Environmental Surfaces  (5). The  9

inclusivity/exclusivity study, robustness testing and matrix studies for all claimed matrixes were  10

conducted independently by Q Laboratories, Inc. (Cincinnati, OH). Product consistency and  11

12

stability data will be submitted when available. 

13

14

Inclusivity/Exclusivity Testing 

15

16

The objective of this experiment was to determine the ability of the 3M Petrifilm Rapid E. 

coli /Coliform Count Plate method to detect a variety of E. coli and non‐ E. coli  coliform strains. A  17

total of 50 E. coli  isolates and 50 non‐ E. coli  coliform isolates were cultured in ISO Buffered  18

peptone water (BPW), prepared per ISO 6579 (6), at 37 ± 0.5°C for 24 ± 2 h.   19

20

The purpose of exclusivity testing was to determine the ability of the 3M Petrifilm Rapid E. 

coli /Coliform Count Plate method to discriminate target organisms from non‐target organisms.  21

12

December 6, 2018