AOAC CASP SMPRs for Comment

1 DRAFT AOAC SMPR 2019.XXX; Version 5; July 16, 2019

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Method Name:

Quantitation of cannabinoids in plant materials of hemp (low THC varieties

Cannabis spp.)

Intended Use : 7 8 1. Purpose: AOAC SMPRs describe the minimum recommended performance characteristics to be 9 used during the evaluation of a method. The evaluation may be an on-site verification, a single- 10 laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC 11 Stakeholder Panels composed of representatives from the industry, regulatory organizations, 12 contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by 13 AOAC Expert Review Panels in their evaluation of validation study data for method being considered 14 for Performance Tested Methods or AOAC Official Methods of Analysis , and can be used as 15 acceptance criteria for verification at user laboratories. Consensus-based Reference method.

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2. Applicability :

The method will be able to identify, and quantify individual cannabinoids (as listed in Table 1a and Table 1b) in plant materials expressed on a dry weight basis. Method must be able to report total

THC regardless of how it is measured (total THC as defined in this SMPR).

3. Analytical Technique :

Any analytical technique(s) that measures the analytes of interest and meets the following method

performance requirements is/are acceptable.

4. Definitions :

Hemp Plant Materials

Fresh or dried, whole or milled plant material of low THC cultivars of Cannabis spp.

Limit of Quantitation (LOQ)

The minimum concentration or mass of analyte in a given matrix that can be reported as a

quantitative result.

Quantitative method

Method of analysis which response is the amount of the analyte measured either directly (enumeration in a mass or a volume), or indirectly (color, absorbance, impedance, etc.) in a certain

amount of sample.

Repeatability

Variation arising when all efforts are made to keep conditions constant by using the same instrument and operator and repeating during a short time period. Expressed as the repeatability

standard deviation (SD r

); or % repeatability relative standard deviation (%RSD r ).