AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

Validation of A LC-MS/MS Method for Analysis of Vitamin K in I nfant Formula and Adult Nutritional Samples

Objective:

Develop and validate a LC-MS/MS method for quantitation of vitamin K in infant formula and adult nutritional samples

Analyte Scope:

Vitamin K1 cis and trans and total Vitramin K2-7 Vitramin K2-4 .

Matrix Scope:

Infant Formula Samples Adult Nutritional

SPIFAN Samples Used for the Method Validation in this study

Provided to Silliker by SPIFAN on 10/28/13 The listing and details regarding the samples provided in Table 1. Table 1: Details of the samples used in the method validation

Sample #

Sample Name

Lot#ID

Child Formula Powder Infant Elemental Powder

00411RF00 00402RF00 00415RF00 00407RF00

1 2 3 4

Adult Nutritional RTF, High Protein Adult Nutritional RTF, High Fat

SPIFAN Blank Milk Form

Infant Formula RTF, Milk Based

5

SRM 1849a

CLC10-b

6

Adult Nutritional Powder Milk Protein Based Infant Formula Powder Partially Hydrolyzed Milk based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat

11750017V3

7

1172572116

8

117257651Z

9

00394RF00 00412RF00 00403RF00

10 11 12

Child Formula Powder Infant Elemental Powder

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

2