AOAC SPIFAN ERP Meeting Book-March 16, 2016

VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis

evaluated range of 5 to 250 ng/mL. Examples of the calibration curve of vitamin K1 peak area and also peak area as a ratio of peak area of internal std. and also for trans vitamin K1 are provided in Figures 2a, 2b and 2c, respectively. Calibration curve of K2-7 and K2-4 are provided in Figures 3 and 4 respectively.

Vit K1

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y = 2233.7x - 1767.6 R² = 0.9995

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Figure 2a: One of the example of the Calibration curve (linear regression) for vitamin K1, vitamin K1 conc (ng/mL) (X axis) and peak area for respective standard (YAxis) and their linear regression relationship.

Vit K1 (ratio of int std)

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y = 0.161x - 0.133 R² = 0.9996

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Figure 2b One of the example of the Calibration curve (linear regression) for vitamin K1, vitamin K1 conc (ng/mL) (X axis) and peak area ratio of vitamin K1 and internal std for respective standard (Y Axis) and their linear regression relationship.

Silliker Laboratories, Chemistry Research Center, Date: 1/30/15

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