AOAC SPIFAN ERP Meeting Book-March 16, 2016
VitK-01 w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE
Silliker Chem, Res. Center Crete, IL – Validation of a LC-MS/MS Method for Vitamin K Analysis
Table 5 : Reconstitution of the powder samples (for evaluation of the method repeatability/precision/spike recovery evaluation) before the analysis
Powder for Reconstitution (g)
Water Added (g)
Sample #
Sample Name
Lot#ID
Child Formula Powder Infant Elemental Powder
00411RF00 00402RF00 00415RF00 00407RF00
1* 2*
12.00093 12.0037
100 100
Adult Nutritional RTF, High Protein Adult Nutritional RTF, High Fat
3*
4*
SPIFAN Blank Milk Form
Infant Formula RTF, Milk Based
5*
SRM 1849a
CLC10-b
6
12.0364
100
Adult Nutritional Powder Milk Protein Based Infant Formula Powder Partially Hydrolyzed Milk based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat
11750017V3
7
12.0203
100
1172572116
8
12.0076
100
117257651Z
9
12.0867
100
00394RF00 00412RF00 00403RF00
10 11 12 13 14
12.0461 12.0862 12.00076 12.0036 12.0504
100 100 100 100 100
Child Formula Powder Infant Elemental Powder
Infant Formula Powder Milk Based Infant Formula Powder Soy Based
D04HTCVV09
E29JVL05
SPIFAN Control Milk Form
Infant Formula RTF Milk Based
15
Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat
00414RF00 00406RF00
16 17
* Samples used for spike recovery evaluation.
Averages and Precision of within day Replicates analysis of SPIFAN sample tested for separate three days: Beverages prepared from the respective powdered samples and the RTF samples (as is) were analyzed for all the targeted vitamin K compounds. The Vitamin K compounds in each powder sample was analyzed in the reconstituted liquid sample. The Results of the averages and repeatability/precision (%RSD r ) for each of the sample for every day of the analysis are calculated. The results are presented in the following Table 5 a-c.
Silliker Laboratories, Chemistry Research Center, Date: 1/30/15
15
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