AOAC SPIFAN ERP Meeting Book-March 16, 2016

2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

Table 24: Total folate as folic analysis in BCR 485 (dried vegetable powder) and other reference materials

Average Total Folates (mcg/g)

Expected Total Folates (mcg/g)

Sample

Matrix

Stdev

%RSD

n

days

2.293 +/- 0.062

NIST 1849A

infant formulas

2.826

0.2

6.9

23

8

NIST 3233

cereal

15.1 +/- 1.2 3.15 +/- 0.28

14.642

1.1 0.3 0.1

7.3

24 25 36

8 9

BCR 485

mixed vegetables

3.05

10.3

LCS Material

cereal

-

0.678

7.4

12

Analysis of the SPIFAN Matrices:

Samples:

A kit of all of the SPIFAN samples was received from SPIFAN in March of 2014. Sample Details are provided on page 1 and reproduced here for the convenience: Provided to Silliker by SPIFAN on 3/26/14 1.) Child Formula Powder Lot # 00411RF00 2.) Infant Elemental Powder Lot # 00402RF00 3.) Adult Nutritional RTF, High Protein Lot # 00415RF00 4.) Adult Nutritional RTF, High Fat Lot # 00407RF00 5.) Infant Formula RTF, Milk Based SPIFAN Blank Milk Form The above blank samples are used for the evaluation of spike recovery of folates by the method 6.) SRM 1849A Lot # CLC10-b 7.) Adult Nutritional Powder Milk Protein Based Lot # 11750017V3

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