AOAC SPIFAN ERP Meeting Book-March 16, 2016

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2011.06 (Fol-22) w/SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Silliker Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analysis

Table 26: Results of the SPIFAN samples for total folate calculated as sum of the detected folate compounds and expressed as mcg/100 g of the reconstitute beverage liquid sample or RTF liquid sample. Averages and precision (as % RSD) of replicates analyzed on the same day for separate 3 days (total n=9).

REC'D AS

Analyzed AS

Av. mcg/100g RTF*

Independent Analysis Days

Sample

Matrix

Lot #/ID

Stdev %RSD

n

Breakfast Cereal Sample

Silliker LCS

LCS**

Powder

Powder

65.2

2.9

4.5

9

3

RTF Liquid RTF Liquid

1

Powder

31.8

2.5

7.9

9

3

SRM 1849A

CLC10-b

Adult Nutritional Powder Milk Protein Based

2

Powder

35.6

4.5

12.7

9

3

11750017V3

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based Adult Nutritional Powder Low Fat

RTF Liquid

3

Powder

20.0

2.5

12.5

9

3

1172572116

RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid RTF Liquid

4

Powder

25.3

2.2

8.9

9

3

117257651Z

5

Powder

42.2

4.1

9.7

9

3

00394RF00

6

Powder

24.6

1.8

7.5

9

3

Child Formual Powder

00412RF00

7

Powder

32.6

3.0

9.3

9

3

Infant Elemental Powder

00403RF00

Infant Formula Powder Milk Based Infant Formula Powder Soy Based

8

Powder

21.5

1.5

7.0

9

3

D04HTCVV

9

Powder

22.5

2.4

10.5

9

3

E29JVLV

SPIFAN CONTROL MILK FORM

Infant Formula RTF Milk Based

10

LIQUID

19.5

2.1

10.5

9

3

Adult Nutritional RTF High Protein Adult Nutritional RTF High Fat

11

LIQUID

56.0

5.5

9.9

9

3

00414RF00

12

LIQUID

66.2

6.5

9.8

9

3

00406RF00

* Total Folate Results are calculated based on sum of all the folate compounds in the samples after the conversion of each compound as folic acid. Results are provided as mcg/g per 100 g of RTF **Total Folate Values for Cereal sample is per 100g of the solid sample The results of total folate as folic acid (sum of the detected folate compounds after calculation as folic acid) in the SPIFAN samples are presented in the above Table. The samples were analyzed in triplicates for three separate days. Averages and repeatability value/precision (%RSDr) for within day analysis are presented. The repeatability/precision (% RSDr) with one exception were less than 7% provided in the SMPR for all the SPIFAN samples for all three days of the analysis.

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